The Ambion® kit is for labeling DNA using a random-priming method, which has technical improvements over existing methods including the use of a high-purity, exonuclease-free Klenow and Random Decamers to produce probes with greater than 109 cpm/µg in 10 min reactions. The kit includes sufficient reagents for 100 reactions.
• Fast, 10 min reaction time
• Elimination of Klenow exonuclease activity maximizes specific activity
• Improved labeling kinetics maximizes yields
• Flexible—both -dATP and -dCTP buffers supplied with each kit
• Decamers produce greater primer-template stability
Contents and Storage
Exo– Klenow, 10X Decamer Solution, 5X Reaction Buffer minus dATP, 5X Reaction Buffer minus dCTP, DECAtemplate™ GAPDH-M, and 0.5 M EDTA should be stored at
–20°C. Nuclease-free water may be stored at any temperature.
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