POLY(A)TAILING KIT 1 KIT EACH

• 产品编号：AM1350      品牌：invitrogen       原厂货号：AM1350
• 产品分类：克隆 > 体外转录 > 体外转录
• 应用分类：基因表达分析和基因分型 > 体外转录

描述：

For the polyadenylation of in vitro transcribed RNA to enhance translation initiation efficiency. Sufficient reagents are included for 25 reactions.

• Adds a poly(A) tail of at least 150 nucleotides in length to the 3' termini of RNA
• Enhances translational efficiency in vivo
• Reaction can be adjusted to control for tail length
• Optimized for use with RNA transcripts synthesized using the mMESSAGE mMACHINE™ Kit

The Poly(A) Tailing Kit uses E. coli Poly(A) Polymerase I (E-PAP) to polyadenylate the 3'-termini of in vitro transcribed RNA. Polyadenylation plays an important role in the stabilization of RNA in eukaryotes and enhances the efficiency of translation initiation. In the Poly(A) Tailing Kit, the E-PAP reaction has been optimized so that mRNAs are efficiently tailed with at least 150 adenines. The additional adenine residues confer stability to the mRNA and may increase translational efficiency of in vitro synthesized capped RNA in microinjection and transfection experiments (see Figure).

Accessory Products:
The Poly(A) Tailing Kit is optimized for use with Ambion's mMESSAGE mMACHINE™ High Yield Capped RNA Transcription Kit. mMESSAGE mMACHINE™ Kits are available with T7 (SKU #AM1334), T3 (SKU #AM1338), or SP6 (SKU #AM1330) polymerases.

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