The Ambion® MAXIscript® SP6In VitroTranscription Kit synthesizes RNA probes in just 10 minutes for use in ribonuclease protection assays,in situhybridization, and blot hybridizations. Each kit contains sufficient reagents for 100 reactions.
Features:
• High yields of high specific activity, full-length RNA probes in just 10 minutes • Ideal probe synthesis kit for nuclease protection assays, northern and Southern blots, andin situhybridization • Includes TURBO DNase™ • All kits contain quality-tested DNase/RNase-free reagents
The MAXIscript®In VitroTranscription Kit synthesizes RNA probes with specific activities reaching 1 × 109cpm/µg in just 10 minutes. MAXIscript® Kits are very efficient at synthesizing full-length probes, even at limiting nucleotide concentrations, making them the perfect companion to ribonuclease protection assays. MAXIscript® Kits are also an excellent choice for the synthesis of nonisotopically labeled probes. Labeling can be accomplished post-transcriptionally by using Ambion's BrightStar® Psoralen-Biotin Labeling Kit, or during transcription by incorporating modified nucleotides into the reaction. MAXIscript® Kits can also be used for the synthesis of modest amounts of unlabeled RNA (2–6 µg/20 µl reaction) of up to 2 kb.
Contents and Storage
SP6 Enzyme Mix, 10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, TURBO DNase, Gel Loading Buffer II, and pTRI-b-actin are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
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