AHP987 recognises an epitope within the C-terminal (CT) region of glutamate receptor subunits 2 and 3 (GluR2/3), components of the AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid) group of ionotropic glutamate receptors, which play a key role at excitatory synapses.
The interaction of the C-terminal amino acids (-SVK1) of AMPA receptor GluR2/3 subunits with PDZ domain-containing proteins, has been implicated in the regulation of synaptic targeting and surface expression of AMPA receptors. Three such PDZ domain-containing proteins identified, are glutamate receptor interacting protein 1 (GRIP1), AMPA receptor-binding protein (ABP/GRIP2) and protein interacting with C-kinase 1 (PICK1).
Studies into cerebellar long-term depression (LTD) have indicated the requirement for PKC-regulated interactions between the C-terminal of GluR2/3 and PDZ domain-containing proteins(1,3).
Species Cross-Reactivity
Target Species
Cross Reactivity
Chicken
Expected from Sequence
Human
Yes
Mouse
Expected from Sequence
Zebrafish
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP987 detects a band of approximately 100 kDa in rat brain hippocampus cell lysates.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.
Antiserum Preparation
Antisera to rat GluR2/3 (CT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
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