AHP954 is specific for human CD268, also known as B cell activating factor receptor (BAFF-R).
CD268 is a member of the tumour necrosis factor receptor family, which is expressed by B cells. CD268 plays a role in BAFF induced B cell development and survival, and is thought to be involved in B cell autoimmune diseases.
AHP954 detects a band approximately 20kDa in Western blots of human, mouse and rat spleen lysates.
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
Yes
Rat
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin(1)
Immunoprecipitation
Western Blotting
2ug/ml - 5ug/ml
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to human CD268 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
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