AHP952 recognizes the type II membrane glycoprotein LOX-1 (lectin-like oxidized LDL receptor-1), a 40-50kDa class E scavenger receptor, expressed by endothelial cells, macrophages, activated platelets and smooth muscle cells. LOX-1 binds to a wide range of ligands, including oxidized low-density lipoprotein (oxLDL), hypochlorite modified high-density lipoprotein (HDL), aged/apoptotic cells, activated platelets and bacteria, reflecting its versatile physiological functions.
AHP952 will detect LOX-1 on transfected cells and in recombinant protein reconstitution assays. LOX-1 levels are low in normal resting human tissues so may fall below detectable levels.
Expression of the LOX-1 gene is upregulated by oxLDL and the binding of LOX-1 to oxLDL results in the activation of NF-kappaB. Furthermore, LOX-1 antibodies have been shown to suppress the oxidized HDL (oxHDL) activation of NF-KappaB in endothelial cells, suggesting that this activation may be due to the binding of oxHDL to LOX-1.
Angiotensin II and the inflammatory cytokine Tumour Necrosis Factor alpha (TNFalpha) also invoke an increase in LOX-1 gene expression and studies have focused on its role in endothelial dysfunction(Chen, M.et al.)(Sawamura, T.et al.)and inflammatory diseases such as atherosclerosis(Murphy, J.E.et al.)and rheumatoid arthritis, as well as its affect on CD40/CD40L signaling in both atherosclerosis and human coronary artery endothelial cells (HCAECs)(Li, D.et al.).
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
1/10
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Neat
Western Blotting
1/10 - 1/100
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106cells in 100ul.
Immunohistology
For the best results AbD Serotec recommend HISTAR detection kits (STAR3000A/B/C).
Western Blotting
This antibody detects intact 50kDa LOX-1 and also a prominent 32kDa proteolytic fragment in western blotting.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to human LOX-1 were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG was prepared by affinity chromatography.
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