Goat anti Dog IgG2 polyclonal antibodyspecifically recognises canine IgG2 by electrophoresis and ELISA. No cross reactivity was detected against other canine immunoglobulin classes, isolated immunoglobulin light chains or no immunoglobulin proteins.
Goat anti Dog IgG2 may cross react with IgG2 from other species.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/10,000 - 1/100,000
Flow Cytometry
Immunohistology - Frozen
1/200 - 1/500
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
1/1000 - 1/10,000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to canine IgG2 were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
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