Goat anti Dog IgE polyclonal antibodyrecognises canine IgE. The antibody has been shown to react specifically with canine IgE by immunoelectrophoresis and ELISA. Less than 0.01% cross reactivity was detected to canine IgG1, IgG2, IgA and IgM. Goat anti Dog IgE may cross react with IgE from other species.
This antibody has been reported for use in immunohistology of frozen canine sections.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/100 - 1/10,000
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
1/1000 - 1/10,000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to canine IgE were raised by repeated immunisations of goats with highly purified antigen. Purified IgG prepared by affinity chromatography.
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