AHP921 is specific for the alpha 2 subunit of rat gamma-aminobutyric acid A (GABAA) receptor.
GABAAreceptors are GABA gated chloride channels, which are important mediators of neuronal inhibition in the mammalian central nervous system. Activation of the GABAAsite results in increased intracellular chloride concentration and hyperpolarization of the cell.
GABAAreceptors are pentameric structures assembled from a family of distinct subunits. At least fourteen distinct subunits have been identified, which show distinct patterns of temporal and spatial expression.
GABAAreceptors may be modulated by several drug types including benzodiazepines and barbiturates, and by phosphorylation by protein kinase C (PKC) and protein kinase A (PKA).
Species Cross-Reactivity
Target Species
Cross Reactivity
Bovine
Expected from Sequence
Dog
Expected from Sequence
Human
Expected from Sequence
Monkey
Expected from Sequence
Mouse
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP921 detects a band of approximately 53kDa in rat cortex lysates.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.
Antiserum Preparation
Antisera to rat GABAAreceptor were raised by repeated immunisations of rabbits with highly purified antigen.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
