AHP900 is specific for rat Ets-like protein-1 (ELK-1) when phosphorylated at serine 383.
ELK-1 is a member of the ternary complex factor (TCF) family of transcription factors, which is thought to play a role in mediating gene expression in response to growth factors.
ELK-1 can be phosphorylated at serine 383 and 389 in its C-terminal region in response to the activation of extracellular signal-regulated kinase (ERK) proteins. Phosphorylation of ELK-1 results in an increase in its transcriptional activity, leading to the transcription of growth-related proteins, such as c-Fos.
Species Cross-Reactivity
Target Species
Cross Reactivity
Dog
Expected from Sequence
Human
Expected from Sequence
Monkey
Expected from Sequence
Mouse
Expected from Sequence
Zebrafish
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting(1)
1/1000
(1)
For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP900 detects a band of approximately 46kDa in Western blots.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.
Antiserum Preparation
Antisera to phosphorylated ELK-1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
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