AHP893 is specific for rat calcium/calmodulin kinase II (CaMK II) alpha and CaMK II beta, when phosphorylated at Thr286.
CaMKII is a multi-functional calcium and calmodulin-dependent kinase which has an important role in cell signalling and becomes autonomously active when phosphorylated at Thr286.
Species Cross-Reactivity
Target Species
Cross Reactivity
Human
Expected from Sequence
Mouse
Expected from Sequence
Xenopus
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting(1)
1/1000
(1)
For the detection of phosphoproteins, threonine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP893 detects bands of approximately 50 and 60 kDa in rat brain lysates, corresponding to CaMKII alpha and CaMK II beta respectively.
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.
Antiserum Preparation
Antisera to phosphorylated rat CaMK II were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG was prepared by affinity chromatography.
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