AHP883 is specific for human MLH1 (Homologue 1 ofE. coliMutL Protein).
MLH1 is housekeeping gene, which plays an important role in DNA mismatch repair. In humans, the protein encoded by MLH1 forms at least two dimeric factors with either PMS2 or MLH3. These heterodimers are reported to form larger complexes made up of several other DNA repair proteins, including MSH2 and MSH6.
In addition to DNA mismatch repair, MLH1 is thought to pay a role in the induction of apoptosis in response to DNA damage.
Mutations in MLH1 have been implicated in certain types of colon cancer.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
1/ 50 - 1/ 200
Immunohistology - Paraffin(1)
1/50 - 1/200
Immunoprecipitation
1ug/mg protein - 4ug/mg protein
Western Blotting
1/500 - 1/5,000
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to human MLH1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
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