AHP871 is specific for human eukaryotic translation initiation factor 4E binding protein 1 (4EBP1).
4EBP1 is principally expressed in the cytoplasm, and has a role in translation regulation.
4EBP1 binds to eukaryotic translation initiation factor 4E (eIF4E), which prevents the formation of the active eIF4E complex. Hyperphosphorylation of 4EBP1 results in the release of eIF4E, thus allowing the translation complex to assemble.
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
Yes
Pig
Yes
Rat
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
1/1000 - 1/6000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to human 4EBP1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
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