AHP851B is specific for bovine interleukin-1 beta (IL-1 beta).
IL-1 beta is principally expressed by macrophages, and plays an important immunomodulatory role in the bovine immune response.
AHP851B recognises both native and recombinant bovine IL-1 beta. No detectable cross-reactivity was observed with human, mouse or rat IL-1 beta.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1ug/ml - 10ug/ml
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This anti interleukin-1β:biotin conjugate may be used in a direct ELISA or as the detection reagent in a sandwich ELISA withrecombinant IL-1β(PBP008) as the standard and eitheranti IL-1β:azide free(AHP851Z) oranti IL-1β:purified(MCA1658) as the capture reagents.
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