AHP834 recognises FLVCR2, previously termed Pituitary Growth/Lactation Coupled (PG/LC) Transporter. This molecule is expressed in cells of the pituitary gland that secrete growth hormone and/or prolactin, and also in other cells and tissues involved in calcium homeostasis. The structure of FLVCR2 contains novel hexad repeats that may be important for protein - protein interaction.
There is increasing evidence that FLVCR2 is expressed by pericytes in the brain and may be vital for endothelial function of capillaries.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin(1)
1/50 - 1/200
Immunoprecipitation
Western Blotting
(1)
Fixation in 4% paraformaldehyde is recommended for Immunohistochemistry.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to FLVCR2 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on Protein A.
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