AHP789B is specific for human MIP-3 beta, (Macrophage Inflammatory Protein 3 beta), otherwise known as CCL19, a 77 amino acid CC chemokine expressed at high levels in the thymus and lymph nodes and to a lesser extent in the spleen, kidney, lungs, small intestine and stomach.
MIP-3 beta acts as a chemoattractant for T and B lymphocytes, dendritic cells, NK cells and myeloid progenitor cells, through specific binding to CCR7, a G-protein coupled receptor known to be up-regulated by Epstein-Barr virus infected B cells and herpesvirus infected T cells. A correlation between the response to MIP-3 beta and the level of CCR7 expression and has been revealed in the maturation of dendritic cells (DCs), and increased levels of both MIP-3 beta and CCR7 expression have been shown in bronchoalveolar lavage fluid (BALF) and BALF cells from patients with the granulomatous disorder sarcoidosis, associated predominantly with CD4+ T lymphocyte alveolitis, and hence T cell recruitment.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
0.15 - 0.30ug/ml
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.1 - 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Antiserum Preparation
Antisera to human MIP-3 beta were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
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