AHP787 is specific for human MIP-1 alpha, otherwise known as CCL3, a 69 amino acid member of the CC chemokine family which plays a critical role in haematopoiesis and is shown to signal through the chemokine receptors CCR1, CCR4 and CCR5.
MIP-1 alpha is also involved in host defence against invading pathogens, acting as a regulator of activation and trafficking of inflammatory cells, such as macrophages, NK cells, granulocytes, dendritic cells and CD8+ lymphocytes. The recruitment of inflammatory cells by MIP-1 alpha, has been linked with the pathogenesis of several diseases, including rheumatoid arthritis,H. pyloriassociated gastritis and bacterial meningitis.
The secretion of MIP-1 alpha by CD8+ lymphocytes has been shown to act as a major HIV-suppressive factor (HIV-SF) through binding to CCR5, identified as a coreceptor for HIV-1 target cell entry.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
0.5ug/ml
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.1 - 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Antiserum Preparation
Antisera to human MIP-1 alpha were raised by repeated immunisations of goats with highly purified antigen. Purified IgG prepared by affinity chromatography.
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