AHP774 is specific for human GRO-gamma, otherwise known as CXCL3, a 7.9kDa CXC chemokine and one of three closely related, but distinct GRO gene products, which act as chemoattractants and activators of neutrophils and basophils.
Like GRO-alpha (CXCL1) and GRO-beta (CXCL2), GRO-gamma contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors, though studies have shown a difference in the potency and receptor binding affinity of these three proteins.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
0.5 - 2.0ug/ml
Flow Cytometry
Functional Assays
1.0 - 2.0ug/ml
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin(1)
0.5 - 0.7ug/ml
Immunoprecipitation
Western Blotting
0.1 - 0.2ug/ml
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Antiserum Preparation
Antisera to human GRO-gamma were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
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