AHP655 recognises human haematopoietic cell-specific lyn substrate 1 (HCLS1), a 75KD intracellular protein involved in the signal transduction pathways in lymphocytes. Expression of HCLS1 is restricted to cells of haematopoietic origin and is expressed in early stages of myeloid and erythroid differentiation.
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
2ug/ml - 4ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP655 detects a band of approximately 70-75kDa in Raji cell lysates. An overnight incubation time of 12 hours is recommended for this antibody.
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to human HCLS1 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
(A full Health and Safety assessment is available upon request) This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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