AHP635 recognises the calcitonin receptor in human, mouse and rat. The calcitonin receptor is a seven transmembrane domain cell surface protein which is coupled to G protein messenger systems. AHP635 recognises an epitope within the cytoplasmic domain that is common to both C1a and C1b rat isoforms.
Calcitonin receptor is expressed in kidney, central and peripheral nervous systems and in osteoclasts. It is also expressed in a number of tissues during the development of the embryo and may be important in foetal morphogenesis.
Species Cross-Reactivity
Target Species
Cross Reactivity
Atlantic Stingray
Yes
Fish
Yes
Human
Yes
Mouse
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/10,000
Flow Cytometry
Immunofluorescence
Immunohistology - Frozen
1/10 - 1/50
Immunohistology - Paraffin(1)
1/10 - 1/50
Immunoprecipitation
Western Blotting
Reducing conditions
(1)
The use of 4% paraformaldehyde fixation, rather than formalin fixation is recommended.
A sensitive detection system is also recommended. e.g. A catalysed signal amplification system.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.
Antiserum Preparation
Antisera to calcitonin receptor were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on Protein A.