AHP629 recognises human ORC3L (origin recognition complex subunit 3-like (yeast)) a protein involved in DNA replication origin binding. ORC3L has been shown to co-precipitate with ORC2L in immunoprecipitation studies.
Species Cross-Reactivity
Target Species
Cross Reactivity
Bovine
Expected from Sequence
Chimpanzee
Expected from Sequence
Dog
Expected from Sequence
Fish
Expected from Sequence
Horse
Expected from Sequence
Mouse
Expected from Sequence
Rat
Expected from Sequence
Rhesus Monkey
Expected from Sequence
Xenopus
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.5 ug/ml - 3 ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP629 detects 2 bands of approximately 75 kDa and 35kDa in HeLa cell lysates.
AHP629 detects a band of approximately 75 kDa in HepG2 cell lysates.
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to human ORC3L was raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by ammonium sulphate precipitation followed by affinity chromatography.
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