AHP596 recognises the C-terminal region of human histone deacetylase 3 (HDAC3). Histone acetylation and deacetylation play important roles in modifying chromatin structure and regulating eukaryotic gene expression. HDAC3 has been characterized as a class I HDAC enzyme. HDAC3 is reported to interact with the SMRT corepressor forming a complex which inhibits histone deacetylase activity.
Species Cross-Reactivity
Target Species
Cross Reactivity
Chicken
Expected from Sequence
Dog
Yes
Fish
Expected from Sequence
Hamster
Yes
Horse
Expected from Sequence
Mouse
Expected from Sequence
Rat
Yes
Rhesus Monkey
Expected from Sequence
Xenopus
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
1/2000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to human HDAC3 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
(A full Health and Safety assessment is available upon request) This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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