AHP500GT recognises a glycoprotein designated TGN46, that is localised primarily in the Trans-Golgi network. TGN46 has been reported as being the best available marker for human trans-Golgi network.
TGN46 is a heavily glycosylated protein of around 110-120kD.
Species Cross-Reactivity
Target Species
Cross Reactivity
Primate
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunofluorescence
Immunohistology - Frozen
0.1ug/ml - 1ug/ml
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.1ug/ml - 1ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Immunohistology
Fixation with 3% paraformaldehyde or methanol/acetone is recommended.
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to human TGN46 were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
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