AHP495 recognises the Parkinson disease related protein known as Parkin.
Parkin is associated with an early onset form of autosomal recessive juvenile Parkinson disease. Parkin shows some similarity in structure to ubiquitin, and is expressed in various regions of the brain including the substantia nigra, as well as in other tissues such as heart, testes and skeletal muscle.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
1/300
Immunoprecipitation
Western Blotting
1/5000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
This antibody detects the 53kDa native protein and the 70kDa ubiquitinated protein under reducing conditions in human neuroblastoma cell lysates.
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to human Parkin were raised by repeated immunisation of goats with highly purified antigen.
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