AHP473 recognises the Bcl-X apoptosis related protein, binding to both the Bcl-XL and Bcl-XS forms. No cross-reactivity to other Bcl-2 family members (including Bcl-2, Bax and Mcl-l) is seen.
In Western blotting applications this antibody may be used to differentiate Bcl-XL (a 30kD protein, often appearing as a doublet) from Bcl-XS (a 20kD protein). Bcl-XS lacks a 63 amino-acid region corresponding to residues 126-188 of Bcl-XL.
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin(1)
1/200 - 1/1000
Immunoprecipitation
Western Blotting
1/1000 - 1/2000
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to human Bcl-X were raised by repeated immunisation of rabbits with highly purified antigen.
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