AHP453B is specific for human fractalkine, otherwise known as CX3CL1, a 397 amino acid CX3C chemokine which signals through the CX3CR1 receptor and is expressed by various tissues including the brain, testis, lung, heart, kidney, colon and skeletal muscle.
Adhesion of fractalkine to the CX3CR1 receptor triggers the chemotaxis and adhesion of leucocytes, including T cells, monocytes and NK cells and studies have suggested that fractalkine is involved in the regulation of brain microglia during neuroinflammatory processes, seeSunnemark, D.et al.for details. CX3CL1 may also play a role in dendritic cell–mediated host defense against invading pathogens such asLegionella pneumophila, seeKikuchi, T.et al.for details.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
0.15 - 0.30ug/ml
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.1 - 0.2ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Antiserum Preparation
Antisera to human fractalkine were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
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