AHP423 recognises ovine interleukin-1 beta (IL-1 beta). No cross-reactivity is seen with ovine IL-6, IL-8, MCP-1 or TNF alpha.
AHP423 neutralises the bioactivity of ovine IL-1 beta. Removal of sodium azide is recommended prior to use in functional assays.
Species Cross-Reactivity
Target Species
Cross Reactivity
Bovine
Expected from Sequence
Goat
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/500
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to ovine IL-1 beta were raised by repeated immunisation of rabbits with highly purified antigen.
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