AHP373 blocks with dynorphin 1-17 and 1-8 but not with other related peptides. Cells are found in the paraventricular and supraoptic nucleus and fibers are found in the lateral hypothalamus in rat brain of 4% FA perfused colchicine tissue.
Species Cross-Reactivity
Target Species
Cross Reactivity
Pig
Yes
Rat
Yes
Rhesus Monkey
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
1/100 - 1/1500
Immunohistology - Paraffin(1)
Immunoprecipitation
Radioimmunoassays
1/10,000
Western Blotting
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Overnight incubation is recommended for paraffin sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to Dynorphin A 1-17 were raised by repeated immunisation of rabbits with highly purified antigen. Whole serum is supplied - diluted.
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