AHP369 recognises neuropeptide E (Glu) I(Ile)-NH2 (NE1-NH2) which is located in the melanin-concentrating hormone (MCH precursor and is co-synthesized with MCH Cells and fibres can be seen in the alpha 2 system of rat hypothalamus perfused with 4% formaldehyde.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
1/200 - 1/2000
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to NEI-NH2 were raised by repeated immunisation of rabbit with highly purified antigen. Whole serum is supplied diluted.
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