AHP362G is specific for human NT-4 (Neurotrophin-4/-5), a 130 amino acid homodimeric cysteine-knot protein and member of the NGF Beta family, expressed highly by the prostate and to a lesser extent by the thymus, skeletal muscle and placenta.
NT-4 is a neurotrophic growth factor structurally related to Beta-NGF, BDNF and NT-3, involved in the development and survival of neurons, through binding to the tyrosine kinase receptor trkB.In vitrostudies have revealed the formation of structurally stable NT4/BDNF and NT4/NGF heterodimers, although their functionin vivohas yet to be determined.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
0.5 ug/ml
Flow Cytometry
Functional Assays
2.0ug/ml - 4.0ug/ml
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
0.1ug/ml - 0.2ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution
Antiserum Preparation
Antisera to human NT-4 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by antigen affinity chromatography.
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