AHP2254 specifically recognises human CCAR1 (Cell cycle and apoptosis regulatory protein 1), a perinuclear phosphoprotein expressed by several epithelial cancer cell lines, which has been implicated as a regulator of transcription, through association with components of the Mediator and p160 coactivator complexes, and its role as a p53 coactivator.
CCAR1 acts as an inducer of apoptosis signalling, under the regulation of the epidermal growth factor receptor (EGFR), caspase-9 activation, the mitogen-activated protein kinase pathway, and in response to chemotherapeutic agents, and has been shown to act as a suppressor of human breast cancer growth.
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
1 - 4ug/mg lysate
Western Blotting
1/2000 - 1/10000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP2254 detects a band of approximately 133-140kDa in HeLa nuclear extract cell lysates.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to human CCAR1 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
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