AHP2225 recognises Rat NEDD4, a cytoplasmic and cell membrane protein with a molecular weight of approximately 102kDa.
NEDD4 is ubiquitously expressed with highest levels being found in lung, kidney and brain.
Species Cross-Reactivity
Target Species
Cross Reactivity
Human
Yes
Mouse
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Functional Assays
Immunodiffusion
Immunohistology - Frozen
Immunohistology - Paraffin
1:100 - 1:200
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Immunohistology
This product does not require protein digestion pre-treatment of paraffin sectionseg. typsin or pronase prior to staining.
This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to rat NEDD4 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
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