AHP2186B specifically recognises human Parathyroid hormone related peptide (PTHrP), a ubiquitously produced neuroendocrine polypeptide hormone, which signals through Parathyroid Hormone Receptor 1 (PTHR1), and acts as a critical regulator of cellular and tissue development, cell differentiation and migration, calcium and phosphorous extracellular concentrations, bone, teeth, and mammary gland development, and epithelial calcium ion transport.
Studies have shown the involvement of PTHrP during the metastasis of colon cancer cells, identifying a positive correlation between PTHrP, Rac1 activity, the guanine nucleotide exchange factor Tiam1, and integrin alpha6beta4. Elevated expression of PTHrP is most commonly associated with breast cancer, and also with gastrointestinal (GI) tumours.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
0.25 - 1.0ug/ml
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.1 - 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Antiserum Preparation
Antiserum to human PTHrP was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
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