AHP2142 specifically recognises human Transglutaminase 2 (TGM2), also known as tissue Transglutaminase (tTG), a ubiquitously expressed calcium-dependent enzyme, with GTPase activity, which catalyzes protein cross-linking and the conjugation of polyamines to proteins.
TGM2 has been implicated in several processes, including apoptosis, the regulation of cytoskeletal structure, cell-matrix adhesion, cell migration, and wound healing. Antibodies against tTG are apparent in a high majority of patients with the autoimmune condition known as coeliac disease, resulting in sensitivity to gluten.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/128000 -
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.03 - 0.1ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP2142 detects a band of approximately 90kDa in human lung cell lysates.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to human TGM2 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
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