AHP2139 specifically recognises human TR3 orphan receptor, otherwise known as NUR77, an immediate-early orphan nuclear receptor, and member of the nuclear receptor hormone family, encoded by the NR4A1 gene.
TR3 orphan receptor plays a role in the mediation of macrophage inflammatory responses, and in apoptosis and cell proliferation, through the binding to a variety of response elements. TR3 orphan receptor has been shown to bind to the anti-apoptotic protein Bcl-2, and can be induced by both physiological and physical stress-related stimuli, including tumour necrosis factor (TNF), inflammatory cytokines and magnetic fields.
AHP2139 is expected to recognise isoform a of TR3 orphan receptor.
Species Cross-Reactivity
Target Species
Cross Reactivity
Dog
Expected from Sequence
Mouse
Expected from Sequence
Rat
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/32000 -
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
1.0 - 3.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP2139 detects a band of approximately 60kDa in human thyroid gland cell lysates.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to human TR3 orphan receptor was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
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