AHP2129 specifically recognises an epitope within the C-terminal (CT) region of human clusterin, otherwise known as Apolipoprotein J, a ubiquitously expressed glycoprotein and member of the clusterin family, implicated in apoptosis (programmed cell death), cell-cell adhesion, DNA repair, membrane recycling, spermatogenesis, and the inhibition of complement-mediated cell lysis.
Clusterin is a component of the SC5b-9 complex which plays a role in inhibiting the complement cascade, through the prevention of the binding of a C5b-C7 complex to the membrane of the target cell. Clusterin exists as both a pro-apoptotic form localised to the nucleus, and a pro-survival secreted form, and has been detected at elevated levels in Alzheimer’s disease, atherosclerosis, myocardial infarction, and various cancers, including bladder, prostate, and breast tumours.
Species Cross-Reactivity
Target Species
Cross Reactivity
Dog
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/32000 -
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.3 - 1.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP2129 detects a band of approximately 35-37kDa in human colon, kidney and prostate cell lysates.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to human clusterin (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
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