Goat anti Human Desminrecognizes an epitope within the C-terminal (CT) region of human Desmin, a type III intermediate filament (IF), localized near the Z line in sacromeres of skeletal, smooth and cardiac muscle tissue.
Desmin plays an essential role in the architecture of muscle cells, connecting the Z-disk to the subsarcolemmal cytoskeleton, and the contractile apparatus to the, mitochondria, cell nucleus and post-synaptic areas of motor endplates. Desmin is an early indicator for muscle tissue in embryogenesis, and a marker for tumours of myogenic origin. Mutations in the Desmin gene, are responsible for the neuromuscular disorder known as desmin-related cardio skeletal myopathy (CSM), characterized by skeletal muscle weakness.
Species Cross-Reactivity
Target Species
Cross Reactivity
Bovine
Expected from Sequence
Dog
Expected from Sequence
Mouse
Yes
Pig
Expected from Sequence
Rat
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/2000 -
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin(1)
3.0 - 6.0ug/ml
Immunoprecipitation
Western Blotting
0.1 - 0.3ug/ml
(1)
This product requires heat-mediated antigen retrieval prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Goat anti Human Desmindetects a band of approximately 55kDa in human skeletal muscle cell lysates.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to human Desmin (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
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