AHP2109 specifically recognises human COG7 (conserved oligomeric Golgi complex subunit 7), one of eight components (COG1-COG8) of the conserved oligomeric Golgi complex, essential for Golgi apparatus localization, structure, and intracellular protein transport.
Mutations in COG7 are responsible for the congenital disorder of glycosylation type 2E (CDG2E), caused by a defect in protein N-glycosylation, an enzymatic process essential during embryonic development, differentiation, and maintenance of cell function.
Species Cross-Reactivity
Target Species
Cross Reactivity
Dog
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/32000 -
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.03 - 0.1ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP2109 detects a band of approximately 85kDa in HeLa, Jurkat and MOLT4 cell lysates.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to human COG7 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
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