AHP2108 specifically recognises an epitope within the N-terminal (NT) region of human DPM1, otherwise known as dolichol-phosphate mannosyltransferase, a member of the glycosyltransferase 2 family, expressed in the endoplasmic reticulum (ER).
DPM1 is involved in the formation of dolichol phosphate mannose (Dol-P-Man), through the transfer of mannose from GDP-mannose to dolichol monophosphate. Dol-P-Man then acts as a mannosyl donor for protein N-glycosylation, glycosyl phosphatidylinositol membrane anchoring, and O-mannosylation. Defects in DPM1 are responsible for the congenital disorder known as glycosylation type 1E (CDG1E), a metabolic deficiency in glycoprotein biosynthesis, resulting in severe mental and psychomotor retardation.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/32000 -
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.1 - 0.3ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP2108 detects a band of approximately 28kDa in human liver cell lysates.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to human DPM1 (NT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
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