AHP2076 specifically recognises an epitope within the C-terminal (CT) region of human PERP (p53 apoptosis effector related to PMP-22), a novel member of the PMP-22/gas3 family of tetraspan transmembrane proteins, and a direct transcriptional target gene of p53, which is highly expressed by cells undergoing p53-dependent apoptosis.
PERP is a component of intercellular desmosome adhesion junctions, thereby playing an important role in maintaining tissue integrity and in cell-cell adhesion. Down-regulation of PERP has shown correlation with the metastatic capacity of human melanoma cell lines, suggesting a role for PERP as a tumour suppressor.
Species Cross-Reactivity
Target Species
Cross Reactivity
Dog
Expected from Sequence
Mouse
Expected from Sequence
Rat
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/8000 -
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
1.0 - 3.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP2076 detects a band of approximately 20kDa in human liver cell lysates.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to human PERP (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.