AHP2072 specifically recognises human BMCC1, otherwise known as prune homolog 2 (PRUNE2), a cytoplasmic protein highly expressed in the central nervous system and adrenal gland, which is induced during the G1 phase of the cell cycle, and increasingly expressed during nerve growth factor (NGF)-depletion-induced apoptosis.
BMCC1 is highly expressed by certain tumours including noneuroblastomas, melanomas and rhabdomyosarcomas, and may act as an important regulator of tumour cell differentiation, aggressiveness and survival.
Species Cross-Reactivity
Target Species
Cross Reactivity
Dog
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/32000 -
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
1.0 - 3.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP2072 detects a band of approximately 30kDa in human cerebellum cell lysates
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to human BMCC1 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
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