AHP2065 specifically recognises an epitope within the N-terminal (NT) region of human IFIH1 (interferon-induced helicase C domain-containing protein 1), otherwise known as MDA5, a widely expressed cytoplasmic RNA helicase, which acts as a mediator of the innate immune response to viral RNA, and is implicated in apoptosis and growth suppression.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/32000 -
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin(1)
10ug/ml -
Immunoprecipitation
Western Blotting
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Tris/EDTA buffer pH 9.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to human IFIH1 (NT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
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