AHP2061 specifically recognises an epitope within the C-terminal (CT) region of human UBE2C, otherwise known as UBCH10, a member of the ubiquitin-conjugating enzyme family, which catalyzes the ubiquitination of target proteins.
UBE2C is essential for the destruction of mitotic cyclins, and is a central factor of the anaphase promoting complex/cyclosome (APC/C).
AHP2061 can be used in immunofluorescence studies for the staining of paraformaldehyde fixed mitotic and late G2 phase HeLa cells.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/32000 -
Flow Cytometry
Immunofluorescence
2.5ug/ml -
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
1.0 - 3.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP2061 detects a band of approximately 20kDa in mitotic HeLa cell lysates.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to human UBE2C (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.