AHP2026 specifically recognises an epitope within the C-terminal (CT) region of human HSPB8 (heat shock protein beta-8), otherwise known as HSP22, a monomeric protein and member of the small heat shock protein (HSP20) family, which displays temperature-dependent chaperone activity.
HSPB8 is predominantly expressed in the heart and skeletal muscle, and defects in HSPB8 are responsible for the neuromuscular disorder distal hereditary motor neuronopathy type 2A (HMN2A), and the axonal CMT neuropathy Charcot-Marie-Tooth disease type 2L (CMT2L).
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/32000 -
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.5 - 1.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP2026 detects a band of approximately 20-25kDa in human skeletal muscle cell lysates.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to human HSPB8 (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
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