AHP2024 specifically recognises an epitope within the C-terminal (CT) region of AIF1 (Allograft inflammatory factor 1), otherwise known as IBA1, an actin-binding protein first identified in rat cardiac allografts with chronic rejection, which has been shown to enhance membrane ruffling, actin-bundling activity of LCP1 (lymphocyte cytosolic protein 1) and RAC activation.
AIF1 is expressed by activated monocytes, plays a role in vascular inflammation and may play a role in macrophage activation and function.
AHP2024 is expected to recognise AIF1 isoform 1 and isoform 3.
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
Expected from Sequence
Pig
Expected from Sequence
Rat
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/32000 -
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
1.0 - 3.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP2024 detects a band of approximately 16kDa in rat brain cell lysates.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to human AIF1 (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.