AHP1943 recognises Wilms Tumour 1 (WT1), also known as WT33, a 449 amino acid transcription factor.
WT1 plays an important role in cellular development, in particular in the development of the urogenital system, additionally WT1 functions in both the role of a tumour suppressor and in an oncogenic manner in tumour formation.
Defects in the the WT1 gene result in a range of disease states including Frasier syndrome and Wilms Tumour 1.
WT1 is expressed in the Kidney and a subset of haematopietic cells. At least 7 isoforms of WT1 have so far been recognised.
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
Expected from Sequence
Rat
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Functional Assays
Immunohistology - Frozen
Immunohistology - Paraffin(1)
10ug/ml -
Immunoprecipitation
Western Blotting
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to Human Wilms Tumour 1 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
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