AHP1939 recognizes human Early Growth Response Protein 1, (EGR1), a 57.5 kDa nuclear protein belonging to the EGR family of C2H2-type zinc-finger proteins.
EGR1 functions as a transcriptional regulator. The products of target genes it activates are required for differentiation and mitogenesis. Research has also demonstrated that EGR1 expression is elevated in a range of cancers including prostate cancer where EGR1 has been shown to promote the growth of tumour cells.
Species Cross-Reactivity
Target Species
Cross Reactivity
Bovine
Expected from Sequence
Chimpanzee
Expected from Sequence
Dog
Expected from Sequence
Rhesus Monkey
Expected from Sequence
Sheep
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Functional Assays
Immunohistology - Frozen
Immunohistology - Paraffin(1)
10ug/ml -
Western Blotting
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to Human EGR1 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
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