AHP1937 recognises human Ubiquitin carboxyl-terminal hydrolase 7 (USP-7), also known as HAUSP, a 128 kDa ubiquitin specific protease belonging to the peptidase C19 family.
USP7 is deubiquitylating enzyme that cleaves ubiquitin from its substrates resulting in stabilization of the protein.
USP7 is a direct antagonist of MDM2 and has been demonstrated to protect p53 from degradation by MDM2 and thus implying that USP7 may act in a tumour suppressor role.
Species Cross-Reactivity
Target Species
Cross Reactivity
Bovine
Yes
Chicken
Yes
Chimpanzee
Yes
Dog
Yes
Mouse
Yes
Rat
Yes
Zebrafish
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1:15000 - 1:60000
Flow Cytometry
Functional Assays
Immunohistology - Frozen
Immunohistology - Paraffin
5ug/ml -
Immunoprecipitation
Western Blotting
1:500 - 1:2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Immunohistology
This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to Human USP7 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
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