AHP1920 specifically recognises human CCL25, otherwise known as TECK (thymus expressed chemokine), a member of the intercrine beta (chemokine CC) family, specifically expressed by thymic stromal cells, which acts as a chemotactic factor for activated macrophages, thymocytes and dendritic cells, and signals through binding to the CCR9 receptor.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
0.5 - 2.0ug/ml
Flow Cytometry
Functional Assays
10.0 - 15.0ug/ml
Immunohistology - Frozen
Immunohistology - Paraffin(1)
0.5 - 1.0ug/ml
Immunoprecipitation
Western Blotting
0.1 - 0.2ug/ml
(1)
This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Antiserum Preparation
Antiserum to human CCL25 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
This antibody may be used in an indirect ELISA or as a capture reagent in a sandwich ELISA withAHP1920Bas the detection antibody andPHP225as the standard.
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