AHP1918 specifically recognises platelet factor-4 (PF-4), otherwise known as CXCL4, a member of the intercrine alpha (chemokine CXC) family, released from the alpha granules of activated platelets, during platelet aggregation.
PF-4 binds with high affinity to heparin, counteracting its’ anticoagulant effect, thereby promoting blood coagulation, and implicating PF-4 in wound repair. PF-4 acts as a chemotactic factor for neutrophils, monocytes and fibroblasts, and binds wih CXCR3B, a splice variant of the chemokine receptor CXCR3.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
0.5 - 2.0ug/ml
Flow Cytometry
Functional Assays
1.75 - 3.0ug/ml
Immunohistology - Frozen
Immunohistology - Paraffin(1)
Immunoprecipitation
Western Blotting
0.1 - 0.2ug/ml
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Antiserum Preparation
Antiserum to human PF-4 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
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